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Test Code: CG AMNIO
General Information: Amniotic fluid cells, obtained by transabdominal amniocentesis during the second trimester of pregnancy at 14 to 18 weeks of gestation, are cultured and analyzed by the in situ method. This involves monolayer "in situ" culture on microscope cover slips. Cell cultures are harvested, stained and analyzed without trypsin detachment from the cover slips.
  • Advanced maternal age (> 35 years of age at EDC)
  • Previous child/pregnancy with a chromosome abnormality
  • Family history of an inherited chromosome abnormality
  • Abnormal maternal blood triple marker screen (AChE, UE3, AFP)
  • Abnormalities found on ultrasound examination
  • Known metabolic disorder (inborn error of metabolism) for which prenatal diagnosis is available
  • Known molecular genetic disease for which prenatal diagnosis is available
  • Abnormal mosaic findings on CVS during the first trimester
  • Increased nuchal thickening observed by prenatal ultrasound


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