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Test Code: EMA
Description And Clinical Indication: Recommended to order together with Osmotic Fragility for the diagnosis of hereditary spherocytosis in hematology.

Flow cytometry lab is offering a new test, EMA binding(band 3) to investigate suspected red cell membrane disorder such as hereditary spherocytosis or hereditary pyropoikilocytosis.

EMA is useful in detecting hereditary spherocytosis (HS) in the setting of non-immune spherocytic hemolytic anemia, and shows promise in detecting mild or atypical presentations of HS.

The flow cytometric test measures the fluorescence intensity of intact red cells labeled with the dye eosin-5-maleimide (EMA), which reacts covalently with band 3 protein. The flow method is a reliable, speedy diagnostic test for HS with very good sensitivity and specificity. If the pre-test probability of HS is high, a positive EMA study would confirm the diagnosis. For more complex clinical presentations, EMA together with one or more traditional assays for HS (osmotic fragility, autohemolysis and/or acid glycerol lysis tests) may be useful.


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