||The Stanford Actionable Mutation Panel for Fusions (Fusion-STAMP) is a targeted next generation sequencing method that detects potentially clinically actionable gene fusion events in cancer. The targeted sequencing approach and integrated bioinformatics workflow is optimized for sequencing of formalin fixed paraffin embedded tissue specimens. The workflow includes isolation of total RNA molecules, followed by efficient preparation of sequencing libraries and a target enrichment approach to capture mRNA transcript regions of interest for sequencing. The enrichment is done using custom designed libraries of capture oligonucleotides that target a specific set of expressed genomic regions. This panel fully targets the transcript isoforms of 43 genes which were selected based on their known impact as actionable targets of existing and emerging anti-cancer therapies, their prognostic features, and/or their utility as diagnostic cancer biomarkers. Pooled libraries are sequenced on an Illumina sequencing instrument.
Genes tested by NGS: