T-Cell Receptor Gene Rearrangement Measurable/Minimal Residual Disease Assessment by Next-Generation Sequencing

ORDER CODE: TCRMRD

ORDERING

SPECIMEN

PROCESSING

RESULTS

T cells undergo somatic rearrangement of the T-cell receptor (TCR) genes, resulting in a high diversity of antigen binding capability. TCR is a heterodimeric protein consisting of highly variable α and β chains (αβ T-cells), or γ and δ chains (γδ T-cells). TCR gene rearrangements occur early in T-cell ontogeny and are unique to each T-cell. These rearrangements can therefore be used as a marker of a common origin, or clonality, among a T-cell population, and in the evaluation of measurable/minimal residual disease in lymphoid hematopoietic disorders. The TCR-β (TRB) gene locus is on chromosome 7(7q34) and includes 65 Vβ (variable) gene segments followed by two separate clusters of gene segments; each cluster includes a Dβ (diversity) region, a Jβ (joining) region containing several Jβ gene segments, and a Cβ (constant) region spread over 685 kilobases. The TCR-γ (TRG) gene locus is also on chromosome 7 (7q14) and includes 14 Vγ, 5 Jγ gene, and 2 Cγ gene segments spread over 200 kilobases. The Invivoscribe LymphoTrack assays are targeted next generation sequencing (NGS)-based methods that enable quantitative identification of T-cell populations using a single multiplex master mix of primers targeting the conserved V(D)J regions of the T-cell receptor γ and β (TRG and TRB) genes. The analytical sensitivity by our validation studies is one T-cell equivalent in a background of 100,000 nucleated cells with a DNA input of at least 640ng per replicate.

Test Code

SHC TEST CODE LPCH TEST CODE
LABTCRMRDB (whole blood); LABTCRMRDBM (bone marrow) LABTCRMRDB (whole blood); LABTCRMRDBM (bone marrow)

Specialty

Genetics; Hematology

Synonyms

TCRMRD, MRD, TCR, T-cell

Useful For

Quantitative detection of T cell receptor Gamma (TRG) and/or Beta (TRB) gene rearrangements in minimal residual disease specimens.

Clinical Utility

Quantitative detection of T cell receptor Gamma (TRG) and/or Beta (TRB) gene rearrangements in minimal residual disease specimens.

CPT Code

81479
Collection Instructions
Collect two lavender tops for peripheral blood; collect one lavender top for bone marrow aspirate.

Specimen Quantity and Stability

Special Handling
Ship refrigerated. Do not freeze.
Causes for Rejection
Received frozen, wrong container type

Department

Molecular Pathology

Standard Run Time(s)

Weekly

Turnaround Time

ROUTINE STAT
14 days Not Available

Methodology

Next-generation sequencing
T cells undergo somatic rearrangement of the T-cell receptor (TCR) genes, resulting in a high diversity of antigen binding capability. TCR is a heterodimeric protein consisting of highly variable α and β chains (αβ T-cells), or γ and δ chains (γδ T-cells). TCR gene rearrangements occur early in T-cell ontogeny and are unique to each T-cell. These rearrangements can therefore be used as a marker of a common origin, or clonality, among a T-cell population, and in the evaluation of measurable/minimal residual disease in lymphoid hematopoietic disorders. The TCR-β (TRB) gene locus is on chromosome 7(7q34) and includes 65 Vβ (variable) gene segments followed by two separate clusters of gene segments; each cluster includes a Dβ (diversity) region, a Jβ (joining) region containing several Jβ gene segments, and a Cβ (constant) region spread over 685 kilobases. The TCR-γ (TRG) gene locus is also on chromosome 7 (7q14) and includes 14 Vγ, 5 Jγ gene, and 2 Cγ gene segments spread over 200 kilobases. The Invivoscribe LymphoTrack assays are targeted next generation sequencing (NGS)-based methods that enable quantitative identification of T-cell populations using a single multiplex master mix of primers targeting the conserved V(D)J regions of the T-cell receptor γ and β (TRG and TRB) genes. The analytical sensitivity by our validation studies is one T-cell equivalent in a background of 100,000 nucleated cells with a DNA input of at least 640ng per replicate.

Test Code

SHC TEST CODE LPCH TEST CODE
LABTCRMRDB (whole blood); LABTCRMRDBM (bone marrow) LABTCRMRDB (whole blood); LABTCRMRDBM (bone marrow)

Specialty

Genetics; Hematology

Synonyms

TCRMRD, MRD, TCR, T-cell

Useful For

Quantitative detection of T cell receptor Gamma (TRG) and/or Beta (TRB) gene rearrangements in minimal residual disease specimens.

Clinical Utility

Quantitative detection of T cell receptor Gamma (TRG) and/or Beta (TRB) gene rearrangements in minimal residual disease specimens.

CPT Code

81479

close ORDERING
Collection Instructions
Collect two lavender tops for peripheral blood; collect one lavender top for bone marrow aspirate.

Specimen Quantity and Stability

Special Handling
Ship refrigerated. Do not freeze.
Causes for Rejection
Received frozen, wrong container type

close SPECIMEN

Department

Molecular Pathology

Standard Run Time(s)

Weekly

Turnaround Time

ROUTINE STAT
14 days Not Available

close PROCESSING

Methodology

Next-generation sequencing

close RESULTS