Heme-STAMP, Blood
ORDER CODE: HSTAMPBORDERING
SPECIMEN
PROCESSING
RESULTS
Test Code
SHC TEST CODE | LPCH TEST CODE |
---|---|
LABHSTAMPB | LABHSTAMPB |
Specialty
Synonyms
Clinical Utility
This assay detects single nucleotide variants (SNVs), short insertion-deletions and selected gene fusions in 164 genes recurrently altered in myeloid and lymphoid neoplasms. The Stanford Actionable Mutation Panel for Hematopoietic and Lymphoid Malignancies (Heme-STAMP) is a targeted next generation sequencing method. The workflow includes acoustic shearing of isolated genomic DNA, followed by efficient preparation of sequencing libraries and a targeted enrichment approach to capture genomic regions of interest. The enrichment is accomplished using custom designed libraries of capture oligonucleotides that target a specific set of genomic regions. This panel targets 164 genes, either in part or fully, with the genes selected on the basis of their known impact as actionable targets of existing and emerging anti-cancer therapies, their prognostic features, and/or their mutation recurrence frequency across patients with hematopoietic neoplasms. These genomic features are interrogated to achieve a minimum analytic detection-limit of 5% for SNVs and insertion-deletion variants. Pooled libraries are sequenced on an Illumina sequencing instrument.
Due to inherent limitations of the NGS method, insertion-deletion variants larger than 25 bp are not reliably detected. To detect larger insertion and deletions in key regions of CALR and FLT3, PCR amplification of these regions is performed, followed by capillary electrophoresis fragment analysis.
CPT Code
Collect blood following standard venipuncture collection procedures.
Volume
REQUESTED VOLUME | MINIMUM VOLUME(PEDIATRIC) |
---|---|
4 mL | 1 mL |
Specimen Stability
TEMPERATURE | |||
---|---|---|---|
SPECIMEN TYPE | ROOM TEMP | REFRIGERATED | FROZEN |
Whole Blood | Not Available | Not Available | Not Available |
Sample should be kept at refrigerator temperatures and arrive in lab within 48 hours of collection. Ship on ice.
Heparin
Department
Methodology
Standard Run Time(s)
Turnaround Time
ROUTINE | STAT |
---|---|
21 days | 21 days |
Methodology
Interpretation
Click to view list of targeted genes: Targeted Genes
Test Code
SHC TEST CODE | LPCH TEST CODE |
---|---|
LABHSTAMPB | LABHSTAMPB |
Specialty
Synonyms
Clinical Utility
This assay detects single nucleotide variants (SNVs), short insertion-deletions and selected gene fusions in 164 genes recurrently altered in myeloid and lymphoid neoplasms. The Stanford Actionable Mutation Panel for Hematopoietic and Lymphoid Malignancies (Heme-STAMP) is a targeted next generation sequencing method. The workflow includes acoustic shearing of isolated genomic DNA, followed by efficient preparation of sequencing libraries and a targeted enrichment approach to capture genomic regions of interest. The enrichment is accomplished using custom designed libraries of capture oligonucleotides that target a specific set of genomic regions. This panel targets 164 genes, either in part or fully, with the genes selected on the basis of their known impact as actionable targets of existing and emerging anti-cancer therapies, their prognostic features, and/or their mutation recurrence frequency across patients with hematopoietic neoplasms. These genomic features are interrogated to achieve a minimum analytic detection-limit of 5% for SNVs and insertion-deletion variants. Pooled libraries are sequenced on an Illumina sequencing instrument.
Due to inherent limitations of the NGS method, insertion-deletion variants larger than 25 bp are not reliably detected. To detect larger insertion and deletions in key regions of CALR and FLT3, PCR amplification of these regions is performed, followed by capillary electrophoresis fragment analysis.
CPT Code
close ORDERING
Collect blood following standard venipuncture collection procedures.
Volume
REQUESTED VOLUME | MINIMUM VOLUME(PEDIATRIC) |
---|---|
4 mL | 1 mL |
Specimen Stability
TEMPERATURE | |||
---|---|---|---|
SPECIMEN TYPE | ROOM TEMP | REFRIGERATED | FROZEN |
Whole Blood | Not Available | Not Available | Not Available |
Sample should be kept at refrigerator temperatures and arrive in lab within 48 hours of collection. Ship on ice.
Heparin
close SPECIMEN
Department
Methodology
Standard Run Time(s)
Turnaround Time
ROUTINE | STAT |
---|---|
21 days | 21 days |
close PROCESSING
Methodology
Interpretation
Click to view list of targeted genes: Targeted Genes
close RESULTS