Flow Cytometry ALPS Screen Panel
ORDER CODE: ALPSORDERING
SPECIMEN
PROCESSING
RESULTS
Test Code
SHC TEST CODE | LPCH TEST CODE |
---|---|
LABALPS | LABALPS |
Specialty
Synonyms
Clinical Utility
T cells play an integral role in adaptive immunity and are broadly defined based on expression of the T cell receptor (TCR), CD4, CD8, and NK markers. TCR is a dimer consisting of alpha and beta (α/ß) chains or gamma and delta (γ/δ) chains. Over 90% of T cells in the periphery express TCRα/ß, which are further divided by the presence of absence of costimulatory receptors CD4 and CD8. Autoimmune lymphoproliferative syndrome (ALPS) is a rare disorder of impaired lymphocyte homeostasis that leads to autoimmunity and increased risk of lymphoma. Patients with ALPS typically present in the early years of life with nonmalignant lymphadenopathy, hepatosplenomegaly, and autoimmune cytopenias. One of the hallmarks of ALPS is expansion of CD4/CD8 double negative (DN) T cells that express TCR α/ß. TCRα/ß+ DN T cells normally constitute less than 1% of peripheral blood and lymph node T cells but are increased in patients with ALPS.This assay quantifies T cell subsets based on expression of CD4, CD8, TCRα/ß, and TCRγ/δ by flow cytometry and assesses for the presence of increased TCRα/ß+ DN T cells
CPT Code
Collect blood following standard venipuncture collection procedures.
Volume
REQUESTED VOLUME | MINIMUM VOLUME(PEDIATRIC) |
---|---|
4 mL Whole Blood | 1 mL Whole Blood |
Specimen Stability
TEMPERATURE | |||
---|---|---|---|
SPECIMEN TYPE | ROOM TEMP | REFRIGERATED | FROZEN |
Whole Blood | 24 hours | 48-72 hours | Not Available |
Transport at room temperature, do not freeze. To be received within 24 hours of collection.
Hemolyzed or clotted specimen; inproperly labeled specimen
Department
Standard Run Time(s)
Turnaround Time
ROUTINE | STAT |
---|---|
72 hours | 72 hours |
Methodology
Components
COMPONENT |
---|
CD3 |
CD4 |
CD8 |
TCR α/β |
TCR γ/δ |
CD45 |
Test Code
SHC TEST CODE | LPCH TEST CODE |
---|---|
LABALPS | LABALPS |
Specialty
Synonyms
Clinical Utility
T cells play an integral role in adaptive immunity and are broadly defined based on expression of the T cell receptor (TCR), CD4, CD8, and NK markers. TCR is a dimer consisting of alpha and beta (α/ß) chains or gamma and delta (γ/δ) chains. Over 90% of T cells in the periphery express TCRα/ß, which are further divided by the presence of absence of costimulatory receptors CD4 and CD8. Autoimmune lymphoproliferative syndrome (ALPS) is a rare disorder of impaired lymphocyte homeostasis that leads to autoimmunity and increased risk of lymphoma. Patients with ALPS typically present in the early years of life with nonmalignant lymphadenopathy, hepatosplenomegaly, and autoimmune cytopenias. One of the hallmarks of ALPS is expansion of CD4/CD8 double negative (DN) T cells that express TCR α/ß. TCRα/ß+ DN T cells normally constitute less than 1% of peripheral blood and lymph node T cells but are increased in patients with ALPS.This assay quantifies T cell subsets based on expression of CD4, CD8, TCRα/ß, and TCRγ/δ by flow cytometry and assesses for the presence of increased TCRα/ß+ DN T cells
CPT Code
close ORDERING
Collect blood following standard venipuncture collection procedures.
Volume
REQUESTED VOLUME | MINIMUM VOLUME(PEDIATRIC) |
---|---|
4 mL Whole Blood | 1 mL Whole Blood |
Specimen Stability
TEMPERATURE | |||
---|---|---|---|
SPECIMEN TYPE | ROOM TEMP | REFRIGERATED | FROZEN |
Whole Blood | 24 hours | 48-72 hours | Not Available |
Transport at room temperature, do not freeze. To be received within 24 hours of collection.
Hemolyzed or clotted specimen; inproperly labeled specimen
close SPECIMEN
Department
Standard Run Time(s)
Turnaround Time
ROUTINE | STAT |
---|---|
72 hours | 72 hours |
close PROCESSING
Methodology
Components
COMPONENT |
---|
CD3 |
CD4 |
CD8 |
TCR α/β |
TCR γ/δ |
CD45 |
close RESULTS