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Immunofluorescence

A Diagnostic Modality Supporting  Anatomic Pathology

Immunofluorescence staining is a technique used in the laboratory to diagnose diseases of the skin, kidney, heart, cilia and other organ systems.

Immunofluorescence utilizes a specific pairing between antigen and antibody to identify the deposition of abnormal molecules and proteins in the tissue or cell culture sample. Immunofluorescence (IF) staining labels a specific target antigen with a fluorescent dye such as fluorescein isothiocyanate or cyanine. The fluorescent stain visualized under a fluorescent microscope allows for an assessment of the target molecule/protein distribution in the sample.

 

Stanford offers both direct immunofluorescence and indirect immunofluorescence, performed by highly qualified staff.

 

Contact Information

Tissue collection for immunofluorescence requires special handling and transport solutions (Zeus/Michel’s Transport Medium). Please contact the lab to request biopsy kits:

Immunofluorescence/Electron Microscopy Lab

650-725-5196

Monday-Friday 8 a.m. to 5 p.m.

 

Related Collection Guides:

  • Renal Biopsy Collection Guide
  • Epidermolysis Bullosa Collection Guide
 
Medical Director:
Megan Troxell, MD, PhD

Megan Troxell, MD, PhD

More information about Megan Troxell

Immunofluorescence Stains

C3
C1q
C4D
Fibrinogen
Fibronectin
Albumin

Amyloid A
Amyloid P
Prealbumin/ Transthyretin
IgA
IgG
IgM

Kappa
Lambda
Type IV Collagen: a2 & a5 cocktail
PLA2R
IgG subtypes: IgG1, IgG2, IgG3 & IgG4

Renal Panels

STANDARD IF PANEL:

IgG, IgM, IgA, C3, C1q, fibrinogen, albumin, kappa and lambda

SPECIAL PANELS:

Alport Diagnosis Panel: Type IV Collagen: a2 & a5 cocktail

Amyloid Panel: Amyloid A, Amyloid P, prealbumin/transthyretin, kappa, lambda

IgG Subtypes: IgG1-4 for MGRS

Membranous Nephropathy:  PLA2R (Membranous Nephropathy)  

Antigen Retrieval IF on Paraffin Embedded Tissue: For inadequate frozen tissue or MGRS

Dermatopathology Panels

EPIDERMOLYSIS BULLOSA PANELS:

Primary panel: Antibodies against collagen XVII (BP180), collagen IV, collagen VII, laminin-332 gamma 2 chain

Secondary panel: Antibodies against laminin-332 beta 3 chain, laminin-332 alpha 3 chain, and beta 4 integrin

Antibodies against keratin 5, keratin 14, and plectin are run on an as-needed basis.

DIF Panel: C3, IgG, IgA, IgM, fibrinogen

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