Stanford Dermatopathology evaluates biopsies for the diagnosis of epidermolysis bullosa (EB) utilizing electron microscopy and utilizing immunomapping of the basement membrane zone.
We provide this service in close collaboration with Stanford’s Pediatric Dermatology service at Stanford and our Clinic for Bullous Disorders, ensuring that a team of experts in this subject are reviewing the case.
Our antibody panel for mapping of the basement membrane zone includes:
mA6K5, anti-keratin 5
mAb K14, anti-keratin 14
mAb HD121, anti-plectin
mAb 123, collagen XVII (BP180)
mAb 3E1, anti-beta 4 integrin
mAb K140, anti-laminin-332 beta 3 chain
mAb D4B5, anti-laminin-332 gamma 2 chain
mAb BM165, anti-laminin-332 alpha 3 chain
mAb AE2, anti-type IV collagen
mAb LH-72, anti-type VII collagen
Primary panel: Antibodies against collagen XVII (BP180), collagen IV, collagen VII, laminin-332 gamma 2 chain
Secondary panel: Antibodies against laminin-332 beta 3 chain, laminin-332 alpha 3 chain, and beta 4 integrin
Antibodies against keratin 5, keratin 14, and plectin are run on an as-needed basis.
Specimen collection for EB requires special handling. Please see our collection guide for more information.